Electroporation Theory
The application of an intense electric field in cell suspension or biological tissue produces pores in the plasmatic membrane; this process is called electroporation or electropermeabilization. The pores facilitate the introduction of molecules into the cell and tissue. Electroporation has applications in the treatment of tumors, genetics, and immunology.
The electroporation dynamics (opening and closing of pores) is not completely understood, in part because electroporation is evaluated by indirect measurements.
A set of possible methods for estimating electroporation could be based on measurements of the electrical properties of the tissues or cells electroporated. These electrical properties have been proposed to verify the electroporation effectiveness in individual cells, cell suspensions and tissues.
Papers:
SUZUKI, D. O.H.; MARQUES, C. M. G. ; RANGEL, M. M. M. Conductive Gel Increases the Small Tumor Treatment With Electrochemotherapy Using Needle Electrodes. Artificial Organs, early view, 2016. [2016AO]
SUZUKI, D. O.H.; ANSELMO, J. ; OLIVEIRA, K. D. ; FREYTAG, J. O. ; RANGEL, M. M. M. ; MARQUES, J. L. B. ; RAMOS, A. Numerical Model of Dog Mast Cell Tumor Treated by Electrochemotherapy. Artificial Organs, v. 39, p. 192-197, 2015. [AO2015]
RAMOS, A. ; SCHNEIDER, A. ; SUZUKI, D. O.H. ; MARQUES, J. L. B. Sinusoidal Signal Analysis of Electroporation in Biological Cells. IEEE Transactions on Biomedical Engineering (Print), v. 59, p. 2965-2973, 2012.[IEEE2012]
SUZUKI, D. O.H. ; RAMOS, A. ; RIBEIRO, M. C. M. ; Cazarolli, L. H. ; SILVA, F. R. M. B. ; LEITE, L. D. ; MARQUES, J. L. B. Theoretical and experimental analysis of electroporated membrane conductance in cell suspension. IEEE Transactions on Biomedical Engineering (Print), v. 58, p. 3310-3318, 2011.[IEEE2011]
RAMOS, A. ; SUZUKI, D. O.H. ; MARQUES, J. L. B. Numerical Simulation of Electroporation in Spherical Cells. Artificial Organs, Blackwell Publishing, Inc., v. 28, n.4, p. 357-361, 2004.